Characterization of a low redox potential laccase from the basidiomycete C30
نویسندگان
چکیده
منابع مشابه
Biochemical characterization of a novel laccase from the basidiomycete fungus Cerrena sp. WR1.
This study reports a new white-rot fungus Cerrena sp. WR1, identified based on an 18S rDNA sequence, which can secrete extracellular forms of laccase with a maximal activity reaching 202 000 U l⁻¹ in a 5-l fermenter. A laccase protein, designated Lcc3, was purified and shown to be N-linked glycosylated by PNGase F and liquid chromatography tandem mass spectrometry analyses. The respective full-...
متن کاملMolecular characterization of laccase genes from the basidiomycete Coprinus cinereus and heterologous expression of the laccase lcc1.
A laccase from Coprinus cinereus is active at alkaline pH, an essential property for some potential applications. We cloned and sequenced three laccase genes (lcc1, lcc2, and lcc3) from the ink cap basidiomycete C. cinereus. The lcc1 gene contained 7 introns, while both lcc2 and lcc3 contained 13 introns. The predicted mature proteins (Lcc1 to Lcc3) are 58 to 80% identical at the amino acid lev...
متن کاملCharacteristics of Three Laccase Isoforms from the Basidiomycete Pleurotus nebrodensis
Figure S1. Cont. 68 PnLac1 95 PoLac 95 PsLac 95 PpLac 95 LsLac 65 HmLac . M M M M . . V V V V . . L L L L . . S S S S . . T T T T . . K K K K . . L L L L . . A A V V . . A A A A . . L L L L . . V V V V . . A A A A . . S S S S . . L L L L . . P P P P . . F F F F . . V V V V . . L L F F . . A A A A . . A A V V . . T T T T . . K K K K . . K K K K . . L L L L . . D D D D . . F F F F . . H H H H . ....
متن کاملA High Redox Potential Laccase from Pycnoporus sanguineus RP15: Potential Application for Dye Decolorization
Laccase production by Pycnoporus sanguineus RP15 grown in wheat bran and corncob under solid-state fermentation was optimized by response surface methodology using a Central Composite Rotational Design. A laccase (Lacps1) was purified and characterized and the potential of the pure Lacps1 and the crude culture extract for synthetic dye decolorization was evaluated. At optimal conditions (eight ...
متن کاملChemical Modifications of Laccase from White-Rot Basidiomycete Cerrena unicolor
Laccases belong to the group of phenol oxidizes and constitute one of the most promising classes of enzymes for future use in various fields. For industrial and biotechnological purposes, laccases were among the first enzymes providing larger-scale applications such as removal of polyphenols or conversion of toxic compounds. The wood-degrading basidiomycete Cerrena unicolor C-139, reported in t...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2002
ISSN: 0014-2956
DOI: 10.1046/j.1432-1033.2002.03324.x